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    ATCC cell culture human umbilical vein endothelial cells huvecs
    Cell Culture Human Umbilical Vein Endothelial Cells Huvecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4929 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/umbilical+vein+endothelial+cell+culture+huvec/Primary+Umbilical+Vein+Endothelial+Cells%3B+Normal%2C+Human/pm42106854-87-0-12
    Average 99 stars, based on 4929 article reviews
    cell culture human umbilical vein endothelial cells huvecs - by Bioz Stars, 2026-09
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    Comparison:

    Article Title: DNA vector for targeted gene therapy
    Article Snippet: Comparison of levels of accumulation of a therapeutic gene, e.g. the green fluorescent protein (GFP) in each cell line: in primary human skeletal myoblast cell culture HSkM (ThermoFisher Scientific #A12555), in primary human dermal fibroblast cell culture HDFa (ATCC® PCS-201-012TM), in primary umbilical vein endothelial cell culture HUVEC (ATCC® PCS-100-013TM), in human osteosarcoma cells MG-63 (ATCC® CRL-1427TM), in human tracheal epithelial cell line CFTE29o- (Collection of Institute of Cytology RAS RF), in human neuroblastoma cell culture SH-SY5Y (ATCC® CRL-2266TM), in primary human kidney epithelial mixed cell culture HREC (ATCC® PCS-400-012TM), in human primary bone marrow cell culture (ATCC® PCS-800-012TM), in primary human peripheral blood mononuclear cell culture PBMC (ATCC® PCS-800-011TM), in human peripheral blood macrophages SC (ATCC®CRL-9855TM), in pancreatic adenocarcinoma cells Panc 10.05 (ATCC® CRL-2547TM) 48 hours after their transfection with each gene therapy DNA vector: gene therapy DNA vector GDTT1.8NAS1-eGFP, gene therapy DNA vector GDTT1.8NAS2-eGFP, gene therapy DNA vector GDTT1.8NAS3-eGFP, gene therapy DNA vector GDTT1.8NAS4-eGFP, gene therapy DNA vector GDTT1.8NAS5-eGFP, gene therapy DNA vector GDTT1.8NAS6-eGFP, gene therapy DNA vector GDTT1.8NAS7-eGFP, gene therapy DNA vector GDTT1.8NAS8-eGFP, gene therapy DNA vector GDTT1.8NAS9-eGFP, gene therapy DNA vector GDTT1.8NAS10eGFP, gene therapy DNA vector GDTT1.8NAS11-eGFP. .. Comparison of levels of accumulation of a therapeutic gene, e.g. the green fluorescent protein (GFP) in each cell line: in primary human skeletal myoblast cell culture HSkM (ThermoFisher Scientific #A12555), in primary human dermal fibroblast cell culture HDFa (ATCC® PCS-201-012TM), in primary umbilical vein endothelial cell culture HUVEC (ATCC® PCS-100-013TM), in human osteosarcoma cells MG-63 (ATCC® CRL-1427TM), in human tracheal epithelial cell line CFTE29o- (Collection of Institute of Cytology RAS RF), in human neuroblastoma cell culture SH-SY5Y (ATCC® CRL-2266TM), in primary human kidney epithelial mixed cell culture HREC (ATCC® PCS-400-012TM), in human primary bone marrow cell culture (ATCC® PCS-800-012TM), in primary human peripheral blood mononuclear cell culture PBMC (ATCC® PCS-800-011TM), in human peripheral blood macrophages SC (ATCC®CRL-9855TM), in pancreatic adenocarcinoma cells Panc 10.05 (ATCC® CRL-2547TM) 48 hours after their transfection with each gene therapy DNA vector: gene therapy DNA vector GDTT1.8NAS1-eGFP, gene therapy DNA vector GDTT1.8NAS2-eGFP, gene therapy DNA vector GDTT1.8NAS3-eGFP, gene therapy DNA vector GDTT1.8NAS4-eGFP, gene therapy DNA vector GDTT1.8NAS5-eGFP, gene therapy DNA vector GDTT1.8NAS6-eGFP, gene therapy DNA vector GDTT1.8NAS7-eGFP, gene therapy DNA vector GDTT1.8NAS8-eGFP, gene therapy DNA vector GDTT1.8NAS9-eGFP, gene therapy DNA vector GDTT1.8NAS10eGFP, gene therapy DNA vector GDTT1.8NAS11-eGFP. .. A similar transfection of these cell lines with plasmid vector pEFGP-N1 (Clontech) was made as a reference.

    Cell Culture:

    Article Title: DNA vector for targeted gene therapy
    Article Snippet: Comparison of levels of accumulation of a therapeutic gene, e.g. the green fluorescent protein (GFP) in each cell line: in primary human skeletal myoblast cell culture HSkM (ThermoFisher Scientific #A12555), in primary human dermal fibroblast cell culture HDFa (ATCC® PCS-201-012TM), in primary umbilical vein endothelial cell culture HUVEC (ATCC® PCS-100-013TM), in human osteosarcoma cells MG-63 (ATCC® CRL-1427TM), in human tracheal epithelial cell line CFTE29o- (Collection of Institute of Cytology RAS RF), in human neuroblastoma cell culture SH-SY5Y (ATCC® CRL-2266TM), in primary human kidney epithelial mixed cell culture HREC (ATCC® PCS-400-012TM), in human primary bone marrow cell culture (ATCC® PCS-800-012TM), in primary human peripheral blood mononuclear cell culture PBMC (ATCC® PCS-800-011TM), in human peripheral blood macrophages SC (ATCC®CRL-9855TM), in pancreatic adenocarcinoma cells Panc 10.05 (ATCC® CRL-2547TM) 48 hours after their transfection with each gene therapy DNA vector: gene therapy DNA vector GDTT1.8NAS1-eGFP, gene therapy DNA vector GDTT1.8NAS2-eGFP, gene therapy DNA vector GDTT1.8NAS3-eGFP, gene therapy DNA vector GDTT1.8NAS4-eGFP, gene therapy DNA vector GDTT1.8NAS5-eGFP, gene therapy DNA vector GDTT1.8NAS6-eGFP, gene therapy DNA vector GDTT1.8NAS7-eGFP, gene therapy DNA vector GDTT1.8NAS8-eGFP, gene therapy DNA vector GDTT1.8NAS9-eGFP, gene therapy DNA vector GDTT1.8NAS10eGFP, gene therapy DNA vector GDTT1.8NAS11-eGFP. .. Comparison of levels of accumulation of a therapeutic gene, e.g. the green fluorescent protein (GFP) in each cell line: in primary human skeletal myoblast cell culture HSkM (ThermoFisher Scientific #A12555), in primary human dermal fibroblast cell culture HDFa (ATCC® PCS-201-012TM), in primary umbilical vein endothelial cell culture HUVEC (ATCC® PCS-100-013TM), in human osteosarcoma cells MG-63 (ATCC® CRL-1427TM), in human tracheal epithelial cell line CFTE29o- (Collection of Institute of Cytology RAS RF), in human neuroblastoma cell culture SH-SY5Y (ATCC® CRL-2266TM), in primary human kidney epithelial mixed cell culture HREC (ATCC® PCS-400-012TM), in human primary bone marrow cell culture (ATCC® PCS-800-012TM), in primary human peripheral blood mononuclear cell culture PBMC (ATCC® PCS-800-011TM), in human peripheral blood macrophages SC (ATCC®CRL-9855TM), in pancreatic adenocarcinoma cells Panc 10.05 (ATCC® CRL-2547TM) 48 hours after their transfection with each gene therapy DNA vector: gene therapy DNA vector GDTT1.8NAS1-eGFP, gene therapy DNA vector GDTT1.8NAS2-eGFP, gene therapy DNA vector GDTT1.8NAS3-eGFP, gene therapy DNA vector GDTT1.8NAS4-eGFP, gene therapy DNA vector GDTT1.8NAS5-eGFP, gene therapy DNA vector GDTT1.8NAS6-eGFP, gene therapy DNA vector GDTT1.8NAS7-eGFP, gene therapy DNA vector GDTT1.8NAS8-eGFP, gene therapy DNA vector GDTT1.8NAS9-eGFP, gene therapy DNA vector GDTT1.8NAS10eGFP, gene therapy DNA vector GDTT1.8NAS11-eGFP. .. A similar transfection of these cell lines with plasmid vector pEFGP-N1 (Clontech) was made as a reference.

    Transfection:

    Article Title: DNA vector for targeted gene therapy
    Article Snippet: Comparison of levels of accumulation of a therapeutic gene, e.g. the green fluorescent protein (GFP) in each cell line: in primary human skeletal myoblast cell culture HSkM (ThermoFisher Scientific #A12555), in primary human dermal fibroblast cell culture HDFa (ATCC® PCS-201-012TM), in primary umbilical vein endothelial cell culture HUVEC (ATCC® PCS-100-013TM), in human osteosarcoma cells MG-63 (ATCC® CRL-1427TM), in human tracheal epithelial cell line CFTE29o- (Collection of Institute of Cytology RAS RF), in human neuroblastoma cell culture SH-SY5Y (ATCC® CRL-2266TM), in primary human kidney epithelial mixed cell culture HREC (ATCC® PCS-400-012TM), in human primary bone marrow cell culture (ATCC® PCS-800-012TM), in primary human peripheral blood mononuclear cell culture PBMC (ATCC® PCS-800-011TM), in human peripheral blood macrophages SC (ATCC®CRL-9855TM), in pancreatic adenocarcinoma cells Panc 10.05 (ATCC® CRL-2547TM) 48 hours after their transfection with each gene therapy DNA vector: gene therapy DNA vector GDTT1.8NAS1-eGFP, gene therapy DNA vector GDTT1.8NAS2-eGFP, gene therapy DNA vector GDTT1.8NAS3-eGFP, gene therapy DNA vector GDTT1.8NAS4-eGFP, gene therapy DNA vector GDTT1.8NAS5-eGFP, gene therapy DNA vector GDTT1.8NAS6-eGFP, gene therapy DNA vector GDTT1.8NAS7-eGFP, gene therapy DNA vector GDTT1.8NAS8-eGFP, gene therapy DNA vector GDTT1.8NAS9-eGFP, gene therapy DNA vector GDTT1.8NAS10eGFP, gene therapy DNA vector GDTT1.8NAS11-eGFP. .. Comparison of levels of accumulation of a therapeutic gene, e.g. the green fluorescent protein (GFP) in each cell line: in primary human skeletal myoblast cell culture HSkM (ThermoFisher Scientific #A12555), in primary human dermal fibroblast cell culture HDFa (ATCC® PCS-201-012TM), in primary umbilical vein endothelial cell culture HUVEC (ATCC® PCS-100-013TM), in human osteosarcoma cells MG-63 (ATCC® CRL-1427TM), in human tracheal epithelial cell line CFTE29o- (Collection of Institute of Cytology RAS RF), in human neuroblastoma cell culture SH-SY5Y (ATCC® CRL-2266TM), in primary human kidney epithelial mixed cell culture HREC (ATCC® PCS-400-012TM), in human primary bone marrow cell culture (ATCC® PCS-800-012TM), in primary human peripheral blood mononuclear cell culture PBMC (ATCC® PCS-800-011TM), in human peripheral blood macrophages SC (ATCC®CRL-9855TM), in pancreatic adenocarcinoma cells Panc 10.05 (ATCC® CRL-2547TM) 48 hours after their transfection with each gene therapy DNA vector: gene therapy DNA vector GDTT1.8NAS1-eGFP, gene therapy DNA vector GDTT1.8NAS2-eGFP, gene therapy DNA vector GDTT1.8NAS3-eGFP, gene therapy DNA vector GDTT1.8NAS4-eGFP, gene therapy DNA vector GDTT1.8NAS5-eGFP, gene therapy DNA vector GDTT1.8NAS6-eGFP, gene therapy DNA vector GDTT1.8NAS7-eGFP, gene therapy DNA vector GDTT1.8NAS8-eGFP, gene therapy DNA vector GDTT1.8NAS9-eGFP, gene therapy DNA vector GDTT1.8NAS10eGFP, gene therapy DNA vector GDTT1.8NAS11-eGFP. .. A similar transfection of these cell lines with plasmid vector pEFGP-N1 (Clontech) was made as a reference.



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    Effect of coating agents on the attachment and growth of Endothelial cells (ECs) on precision cut lung slices (PCLS). ( A ) Schematic diagram describes the experimental design of each assay using decellularized PCLS. ( B , C ) Coating of decellularized scaffolds enhance the attachment of Hoechst 33,342-labeled (blue) mC166 cells on PCLS compared to the uncoated group. Each group n = 6. ( D ) Quantification of EC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 6. ( E ) Quantification of the attachment of Hoechst-labeled HUVECs on anti-CD31 coated PCLS coated with 12.5, 25, 50, or 100 µg/mL anti-CD31 Ab. Results represent the mean of measurements taken from 3 PCLS seeded with HUVEC cells from 3 independent experiments. Each group n = 3. ( F ) Quantification of HUVEC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 3. Scale bar = 200 μm. The results represent the mean of measurements taken from 3 technical replicates. Each number represents a biological replicate (independent experiment). M ean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Journal of Biological Engineering

    Article Title: Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

    doi: 10.1186/s13036-025-00593-x

    Figure Lengend Snippet: Effect of coating agents on the attachment and growth of Endothelial cells (ECs) on precision cut lung slices (PCLS). ( A ) Schematic diagram describes the experimental design of each assay using decellularized PCLS. ( B , C ) Coating of decellularized scaffolds enhance the attachment of Hoechst 33,342-labeled (blue) mC166 cells on PCLS compared to the uncoated group. Each group n = 6. ( D ) Quantification of EC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 6. ( E ) Quantification of the attachment of Hoechst-labeled HUVECs on anti-CD31 coated PCLS coated with 12.5, 25, 50, or 100 µg/mL anti-CD31 Ab. Results represent the mean of measurements taken from 3 PCLS seeded with HUVEC cells from 3 independent experiments. Each group n = 3. ( F ) Quantification of HUVEC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 3. Scale bar = 200 μm. The results represent the mean of measurements taken from 3 technical replicates. Each number represents a biological replicate (independent experiment). M ean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: Endothelial cell growth medium (EGM) (Lonza Bioscience, MD, USA) was used to culture human umbilical vein endothelial cells (HUVEC) (ATCC, Manassas, VA).

    Techniques: Labeling, Activity Assay, Growth Assay, Cell Counting, CCK-8 Assay, Cell Culture